Ed clean operating room air sampling methods
        Clean operating room air samples for microbial contamination of air cleaning technology to take a different level of control, in order to control the cleanliness of the air in the space environment is suitable for all types of surgery requirements. Clean operating room project is a multi-disciplinary, multi-system, multi-functional composition of systems engineering. Clean operating room air purification flow is generally divided into four kinds of forms, namely turbulence type, laminar flow, the secondary flow pattern, mixed type. Using laminar flow technology, subvert the traditional air sampling method, sampling requirements has been greatly improved.
       First, the sampling time:
After disinfection, before the operation sampled.
      Second, the sampling method:
1, distribution:
Indoor area ≤30 m2, located inside the outer diagonal 3:00, pitch inside and outside wall 1m; indoor area> 30 m2, located four corners and center 5:00, square pitch wall 1m.
2, the plate exposure method:
Tablet diameter 9cm, sampling height 1.5m, exposed 5min.
3. Inspection method:
Flat 37 ℃ for 48h. Count the number of colonies and isolated pathogens.
4, the plate exposure method calculation results
50000N
Total number of bacteria (cfu / m3) = A × T, A flat plate area (cm2); T is the exposure time (min); the average number of colonies (cfu) N is
5 results found
(1) I, II class area, the total number of bacteria ≤10cfu / cm3, did not sterilize qualified for the detection of pathogens.
(2) III class region, the total number of bacteria ≤200cfu / cm3, did not sterilize qualified for the detection of pathogens.
(3) IV class area, the total number of bacteria ≤500cfu / cm2, does not sterilize qualified for the detection of pathogens.
       Third, sampling Note:
1, detected in 100 regions, sampling port coping with a flow direction; detected in other regions, sampling ports are up;
2, after sampling medium strip or dish should be at 37 degrees cultured 24h, and calculate the number of colonies, the average number of colonies were selected from a rounded to one decimal place;
3, when using floating planktonic bacteria concentration determination, bacterial try and dust concentration measurement points should be measuring points measured in the same area, and should be in the same position. Each sampling time should not be less than 30 minutes.
4, when the concentration of bacteria measured by sedimentation settling bacteria count that is, to at least try to measure dust concentration measurement points. As an appropriate extension of the settlement period, the number of minimum requirements can reduce the proportion of the dish, but not less than the minimum dust concentration measurement points.
5, no matter what method to detect the concentration of bacteria, there must be two blank. The first dish or medium for the detection strips (each batch a control dish (bar)); the second time during the operation to do controlled trials (per room or per zone will be a control dish (bar)), after opening and immediately cover, simulated operation); twice the control result must be negative, the entire procedure should meet the requirements of aseptic.