Planning and Design of Molecular Biology Laboratory
Detailed introduction:
The main functions of molecular biology laboratory are the basic analysis and operation of molecular biology, including the extraction and identification of DNA from plants, animals and microorganisms, the extraction and identification of RNA from animal tissues, the extraction and identification of plasmid DNA, PCR amplification, molecular hybridization technology, and the construction of molecular libraries. 。 Following is the overall solution for the construction of molecular biology laboratory, which is compiled by Edward Purification.
The overall scheme of molecular biology laboratory construction:
Molecular biology laboratories are usually divided into biochemical laboratories and molecular laboratories. Molecular biology has been developed from the study of the structure of biological macromolecules. Now, more and more studies are still focused on the relationship between the structure and function of biological macromolecules, as well as the mechanism of regulation and control, such as biochemistry, cell biology and genetics. Knowledge of other disciplines is the basis.
1. Biochemical Laboratory Construction Program:
Mainly for scientific research on chemical biology, because there are many chemical reagents, which are harmful to the human body, so the general biochemical laboratory needs a ventilation system, which can discharge harmful gases produced in the experiment outdoors, to ensure that indoor air will not affect the human body, for the three wastes. Processing can not be arbitrary, in accordance with the relevant environmental requirements for processing.
Basic requirements of indoor facilities: biochemical laboratory, pharmaceutical instrument room and office are generally set up. Water supply and drainage, ventilation system, power socket and total power control switch should be set up in the room according to its functional requirements, and leakage protector should be installed. Water and electricity are required for the test bench and the ventilator. There are more than two 220V two-hole and three-hole sockets each. The Office of the experimenter shall not be located in the chemical laboratory.
Pharmaceutical Instrument Room: Fire-proof, moisture-proof and other facilities, a separate storage place for dangerous goods, protective equipment and first aid kits, indoor ventilation and ventilation devices, in general, there is a universal suction hood or atomic absorption hood above the instrument. When using the instrument for experimental operation, exhaust gas can be discharged outdoors, the top of the face medicine cabinet. The department can be equipped with an exhaust system.
Laboratory Furniture: The appearance is neat, the water pipes in the cabinet are not exposed, the countertop can be equipped with faucets and PP sinks, eye washers, drip racks, etc. There should also be power protection devices, with the same size requirements, beautiful color matching, scientific and practical, and the countertop and cabinet body should be kept clean.
2. Molecular Biology Laboratory Construction Program:
The conventional methods of molecular biology research and experiment do not have strict purification requirements, but in order to avoid the possibility of cross-contamination between experimental areas, it is advisable to adopt full-delivery and exhaust air distribution. At the same time, the proportion of air supply and exhaust is controlled to ensure the pressure requirement of each experimental area.
Reagent storage and preparation area: mainly as the preparation of storage reagents, reagent packaging and preparation of the main reaction mixture. Reagents and materials used for sample preparation should be directly transported to this area, not through other areas. Reagent raw materials should be stored in this area and prepared into the required storage reagents in this area. There is no strict requirement for air pressure control in this area.
Specimen preparation area: Preservation of clinical specimens, extraction and storage of nucleic acids (RNA, DNA) and synthesis of DNA when added to amplified reaction tubes and RNA determination. The pressure gradient should be positive relative to the adjacent area to avoid aerosol pollution from the adjacent area. In addition, because aerosol pollution may occur during sampling operation, unnecessary movement in the region should be avoided.
Amplification reaction mixtures preparation and amplification region: mainly DNA amplification, prepared DNA template and synthetic DNA and main reaction mixtures, prepared into reaction mixtures, etc., can also be carried out in this region. In PCR assay, the reaction tube is usually opened after amplification. Therefore, the amplification has a high risk of pollution, so the pressure gradient in this area is required to be negative pressure relative to the adjacent area in order to avoid aerosol leakage from this area.
Amplified Product Analysis Zone: The main operation is the determination of amplified fragments, such as the use of fully automatic closed analyzer detection, this area is not required. Attention should be paid to the pollution sources of the amplified products. Therefore, the pressure gradient in this area is required to be negative pressure relative to the adjacent area in order to avoid the diffusion of the amplified products from this area to other areas.
Laboratory facilities: It is necessary to ensure the laboratory work. According to the different contents of laboratory experiments, corresponding equipment and instruments are equipped, such as ultra-clean worktable, biological cabinet, centrifuge, sample adder, etc.
Shandong Aide Purification Engineering Co., Ltd. is a professional manufacturer of laboratory planning and design, engaged in laboratory decoration, laboratory design, laboratory renovation and other business for many years. The company has a professional laboratory design team, covering the laboratory decoration and construction team. Construction of Microbial Clean Laboratory Design of Microbial Clean Laboratory Decoration of Microbial Clean Laboratory Planning of Microbial Clean Laboratory Find Ed Clean Laboratory. To learn more about the medical purification industry, please pay attention to the Wechat Public No. Aide-188 (Ed Purification and Oxygen Supply)
Previous article:
No more
Next article:
No more