Laminar flow clean operating room environment, the concentration of bacteria and static pressure difference routine monitoring
Laminar flow operating room design and construction standards are becoming increasingly high, so the laminar flow clean operating room routine environmental management and testing to be carried out every day to ensure the effective running of before and after surgery. The following describes the operating room routine monitoring methods:
   1, laminar flow clean operating room routine air monitoring methods:
         It requires daily monitoring of the implementation of dynamic, will test the project for the Tablet sampling method (colony bacteria sedimentation method) or sampling method (planktonic bacteria method) to detect the total number of bacterial colonies.
 (1) Return air dynamic plate sampling method: It should begin in surgery, surgery two hours, before the end of the sampling procedure 3-4 times. Each return air central place three tilt 30 ℃, Φ90 dish, after 30 minutes exposure, 37OC for 24 hours.
Criteria: the average colony counts per dish should conform to Table 4 standards. Single dish should not exceed the average of three times the maximum.
 (2) Dynamic sampling method: planktonic bacteria colonies such testing should be carried out in the surgery incision, suture, between Taiwan and even surgery, for 4 hours, etc., select at least three programs, the total number of bacterial colonies determination:
Standard: Grade I <30cfu / m3; II level <150 cfu / m3; grade III <450 cfu / m3; grade IV <500 cfu / m3.
(3) Other clean buildings at 10 am and 4 pm the same day each measuring 1, placing three Φ90 dish in the middle of each return air, 0.5h after settling for 24h at 37 ℃.
Standard: dynamic plate with return air sampling standards.
(4) Sampling frequency: cleansing operation by the introduction of hospital management control specification execution.
   2, the surface contamination density monitoring methods:
(1) Sampling time: 10 minutes after disinfection (all types of clean space, as a static measured data), after the end of each operating room surgery, and various clean houses 10:00.
(2) Sampling Location: representative place, each room is not less than 2:00 each surface (such as the operating table, tables, lamps, etc.).
(3) The sampling and testing methods: According to "disinfect hospital hygiene standards" (GB15982-1955)
(4) Standard: dynamic plate with return air sampling standards.
   3, health care workers hand sampling: sampling each number less than 3 people
   4, the static pressure differential monitoring methods: pa (Pa)
 (1) Method: The instrument assay.
 (2) Instrument: liquid column manometer decline, the smallest scale 2Pa.
 (3) Personnel: Determination of a man holding the hose and review, a person operate the instrument.
   A, closed: all the doors should be shut down in a clean area, it was the best guard.
   B, Determination: Determination turn outwards from the operating room the highest level of cleanliness innermost room, all interlinked adjacent two chambers can be measured, has been measured to be in communication with the outdoor room. One person in the room should be tested handheld hose extending into the room, so that the nozzle is 0.8m height, nozzle end surface perpendicular to the ground, to avoid the airflow direction and vortex area.
  C, check: If the static pressure is too small and difficult to determine positive and negative, can be used like the thread on the door outside observation.
  D, adjust: If you find the measured pressure does not meet the requirements, you can adjust the valve outlet or switch retest.
(5) Monitoring criteria: I, II class> 8pa; III, IV level> 5 pa; for non-clean areas clean area> 10 pa.
(6) Determination of the number of times: Stay detect and leave the record as required.
  5, the relative humidity monitoring:
  Summer: Do not continuously two days> 60%, can not occur more than twice.
  Winter: 2 consecutive days shall be <30%. It may not occur more than twice.
  To sum up: General laminar flow clean operating room for higher environmental requirements, it is necessary to laminar flow clean operating room bacterial concentration, conventional project hydrostatic poor indicators of daily monitoring to ensure laminar flow clean operating room can Normal safe and effective operation.