The construction of a microbial laboratory is generally composed of six parts: preparation room, washing room, sterilization room, sterilization room, constant temperature culture room and general laboratory. What are the requirements for the design of a microbiology laboratory? Like to do experiments or want to build a laboratory partner and Jin Huatai Xiaobian look down together!
Microbiology laboratories need to meet four functional divisions
(1) Preparatory Room
The preparation room is used for preparing culture medium and sample treatment. There are reagent cabinets, special cabinets for storing utensils or materials, test benches, electric furnaces, refrigerators, upper and lower water channels, power supplies, etc.
(2) Washing room
The washing room is used for washing utensils, etc. Because used utensils have been contaminated by microorganisms, sometimes there are pathogenic microorganisms. Therefore, in the case of conditions permitting, it is best to set up a washing room. Indoors should be equipped with heaters, steamers, basins and barrels for washing utensils, as well as various bottle brushes, decontamination powder, soap, laundry powder, etc.
(3) Sterilization room
Sterilization room is mainly used for sterilization of culture medium and various appliances. Sterilization equipment and facilities such as high-pressure steam sterilizer and oven should be equipped in the room.
(4) Sterile room
The sterile room is usually 4 - 5 square meters, 2.5 meters high, separate from the outside, dedicated to the microbiology laboratory.
Aseptic room, also known as inoculation room, is a special laboratory for systematic inoculation, purification of bacteria and other aseptic operations. In microbial work, inoculation and transplantation of bacteria is a major operation. The characteristics of this operation are to ensure the pure strain of bacteria and prevent the contamination of miscellaneous bacteria. In the air of general environment, because there are many dust and bacteria, it is easy to cause pollution, which greatly interferes with the vaccination work.
The sterile room should be set up according to the principle of economy and science. What are the basic requirements for a good aseptic laboratory to meet its basic requirements?
Four requirements for setting up aseptic laboratories?
(1) The sterile room should have two rooms, one inside the sterile room and the other outside the buffer room. Room volume should not be too large to facilitate air sterilization. The smallest interior area is 2*2.5=5m2, the exterior area is 1*2=2m2, and the height is less than 2.5m. There should be a ceiling.
(2) There should be sliding doors in the interior to reduce the fluctuation of air. The doors should be located farthest from the worktable. The doors in the exterior should also be sliding doors, which should be located farthest from the interior.
(3) A small window should be opened on the walls or "partitions" between the interior and the exterior so as to provide the necessary passageway for the delivery of goods inside and outside during the inoculation process, so as to reduce the number of people entering and leaving the interior and reduce the pollution level. The small window is 60 cm wide, 40 cm high and 30 cm thick, and the inside and outside windows are hung with opposite pull-out fans.
(4) The sterile room has a small and tight volume. After a period of use, the indoor temperature is very high. Therefore, ventilation windows should be set up. The ventilation window should be set on the ceiling at the entrance door of the inner room (that is, the farthest position from the workbench), preferably with double-deck structure, with shutters on the outer layer and suction-plate windows on the inner layer. The ventilation window can be opened after use in the inner room and before sterilization to circulate air. Conditional installation of constant temperature and humidity machine.
Sterile indoor equipment and appliances
(1) The workbench in aseptic room, no matter what material and use, requires smooth surface and level of the workbench.
(2) Install an ultraviolet lamp (mostly 30W) inside and outside. The indoor ultraviolet lamp should be installed directly above the regular working seat, 2 m above the ground. The outdoor ultraviolet lamp can be installed in the center of the outdoor.
(3) the outer rooms should have special work clothes, shoes, caps, masks, pottery basins and towels containing the suer water, hand held sprayers and 5% carbolic acid solutions.
(4) There should be alcohol lamps, commonly used inoculation tools, stainless steel knives, scissors, tweezers, 70% alcohol cotton balls, industrial alcohol, glass sheets, special crayons, notebooks, pencils, label paper, glue, waste baskets and so on.
Sterilization and disinfection of sterile room
(1) Fumigation: This is the measure of sterilizing the sterile room thoroughly. The sterile room should be fumigated and sterilized when it has been used for a long time and the pollution is serious. It can be fumigated with formaldehyde, lactic acid or sulphur.
(2) spray: before the use of asepsis room. Spray can cause particles and microorganisms to settle in the air, preventing dust flying on the desktop and ground, and having bactericidal effect. It can be sprayed with 5% carbolic acid.
(3) Ultraviolet irradiation: before each use of sterile room. Ultraviolet radiation has better bactericidal effect. Usually the ultraviolet lamp should be turned on for 30 to 60 minutes.
Rules of work for aseptic room
(1) Sterilization in sterile room. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5% carbolic acid spray in the interior and exterior chambers.
(2) After washing hands with soap, move the necessary equipment into the outdoor; change the sterilized overalls, hats and shoes into the outdoor, wear a mask, and then soak hands with 2% coal phenol soap for 2 minutes.
(3) move all necessary articles into the inner room for inventory and position. Spray the 5% carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5 ~ 10min.
(4) Before inoculation operation, wipe hands with 70% alcohol cotton ball; during aseptic operation, the movement should be gentle to minimize air fluctuation and dust on the ground.
(5) Attention should be paid to safety in work. If a cotton plug is on fire, hold it tightly with your hand or wrap it in a wet cloth to extinguish it. Never blow it with your mouth to avoid expanding the combustion.