There are only dynamic and static air sampling methods for the environment and daily detection of bacteria in laminar operating cleanroom. The following is an introduction.
1, laminar flow (clean room) static air sampling method:
(1) In the dust-free room, when the air supply outlet is centralized, the operation area and the surrounding area should be detected separately; when the air supply outlet is dispersed, the detection should be carried out according to the unified distribution method of the whole dust-free room.
(2) Colony count (cfu) in the culture dish with diameter of 9 cm was used for sampling. The culture dish was exposed to air for 30 minutes and incubated at 37 for 24 hours. The number of bacteria in the culture dish (cfu) represented the number of bacteria that settled in the air, expressed as one dish per dish.
(3) When sampling by colonial plankton method, the fissure sampler is used to sample the bacteria in the air for 30 minutes at random. The number of colonies (cfu) obtained from the culture of sampling dishes or strips represents the number of bacteria floating in the air, expressed as one/m3.
2. Colonial sampling height:
(1) the sampling points may be placed on the ground or not at any height above the ground 0.8m.
(2) There should be no operating table when the operating area is detected; when the operating table is fixed, the height of detection should be 0.25cm above the table.
3. Point placement method: (see figure below)
4. Notices for Colonial Sampling:
(1) In the 100-level dust-free area, the sampling port should be in the direction of airflow; in other areas, the sampling port should be upward;
(2) Sampled medium strips or dishes should be cultured for 24 hours at 37 C, and the number of colonies should be calculated. The average number of colonies should be rounded to one decimal place.
(3) The sampling time of each colony should not be less than 30 minutes.
(4) No matter what method is used to detect bacterial concentration, there must be two blank controls. For the first time, it was used to detect Petri dishes or culture medium strips (one control dish per batch); for the second time, it was used to control the operation process (one control dish per room or area was opened and immediately sealed for simulated operation); for the second time, the control results must be negative, and the whole operation process should meet the requirements of aseptic operation.
(5) Special tips: when acceptance of new houses, air and surface bacteria should be detected at last.