I. design technical indicators:
A. technical index Description:
The air conditioning system in the experimental area consists of a combined air conditioning unit with a refrigerating capacity of ＊ kW.
Cleanliness: (grade 10000) ≥ 0.5um ≤ 350000 / m3
Microbial plankton: (ten thousand level) ≤ 100 / m3
Sedimentated bacteria: (grade 10000) ≤ 6.25
Lighting: 300lx for the experimental platform and 150lx for other production areas.
The relative static pressure difference between indoor and adjacent rooms is ≥ 5pa, and the relative outdoor static pressure is ≥ 10Pa.
Indoor temperature: 18-26 ℃, indoor relative humidity: 45-60%.
Indoor noise: ≤ 60dB (a).
Power supply: AC220V / 380V-50Hz
B. the air outlet is equipped with a filter, and the air supply outlet is equipped with a filter static pressure box to realize clean air intake. In addition, it is convenient to replace the filter.
C. generally, indoor air supply and exhaust adopts up supply and down exhaust mode. The indoor air outlet shall be set in the area with high indoor pollution risk Zui, and arranged on one side without obstacles.
D. the return air port automatically adjusts the indoor pressure with the residual pressure valve to keep the positive pressure clean.
Operating room purification workshop purification laboratory purification
II. Design Description:
The basic idea of laboratory design is economic and practical. The level of purification requirements is 10000. The laboratory design has the preparation work before the experiment, such as one change, two changes, wind drenching and buffering. In order to reduce experimental pollution and ensure safety, the principle of separation of human flow and logistics is adopted.
A. the layout is compact and reasonable, which meets the requirements of laboratory operation and air cleanliness level. At the same time, it strives to be scientific and economical, and considers the requirements, development and operation cost of the laboratory.
B. the air shower room is set at the entrance of the dust-free room, which can effectively remove the dust carried by the human body and reduce the dust volume of the dust-free room. At the same time, the air shower room also plays the role of airlock to prevent the unclean air from entering the clean area through the door.
C. the clean room needs to supply a certain proportion of fresh air to the room. After purification and filtration, it will enter each laboratory to compensate the exhaust air and ensure the positive pressure and the needs of the staff.
D. the number of air volume return air shall be controlled by the air volume regulating valve in the areas with different requirements of clean level, and the pressure gradient shall be adjusted to ensure that the air flow in the same "clean" area flows to the "polluted" area. The pressure difference between different levels of the dust-free room shall be ≥ 5pa, and the pressure difference between the dust-free room and the outdoor shall be ≥ 10Pa.
E. the dust-free room is equipped with a purple lamp sterilization device.
F. the transfer window is made of all stainless steel with mechanical interlock control and sterilization device.

G. the observation window is airtight and clean, and the door is airtight and clean.

The preparation room, sterilization room, sterile room, constant temperature culture room and general laboratory are composed of five parts. The common features of these rooms are the smooth and hard texture of the floor and walls, the simple furnishing of instruments and equipment, and the convenience of cleaning. The basic requirements of microbiological laboratory are as follows:
(I) preparation room
Preparation room is used for preparation of culture medium and sample treatment, etc. The laboratory is equipped with a reagent cabinet, a special cabinet for storing instruments or materials, an experimental platform, an electric stove, a refrigerator, a sewer, a power supply, etc.
The preparation room is also used for washing utensils, etc. The room should be equipped with heaters, steamers, basins and buckets for washing utensils, as well as various bottle brushes, detergent, soap, washing powder, etc.
(II) sterilization room
The sterilization room is mainly used for the sterilization of culture medium and various instruments. High pressure steam sterilizer, oven and other sterilization equipment and facilities shall be provided in the room.
(III) aseptic room
Aseptic room, also known as inoculation room, is a special laboratory for aseptic operation such as systematic inoculation and purification of bacteria. In the work of microorganism, the inoculation and transplantation of bacteria is a main operation. The characteristic of this operation is to ensure the purity of bacteria and prevent the pollution of mixed bacteria. In the air of general environment, due to the existence of many dust and miscellaneous bacteria, it is easy to cause pollution, which greatly interferes with vaccination work.
1. Setting of aseptic room
Aseptic room should be set up according to the principle of economy and science. The basic requirements are as follows:
(1) there should be two rooms inside and outside the aseptic room. The inside room is the aseptic room and the outside room is the buffer room. The room volume should not be too large for air sterilization. Zui has a small internal area of 2 × 2.5 = 5m2, and an external area of 1 × 2 = 2M2. It is better to have a height of less than 2.5m, with ceilings.
(2) sliding doors shall be set in the inner room to reduce the air fluctuation. The doors shall be set far away from the workbench Zui; the doors in the outer room shall also be set far away from the inner room Zui.
(3) a small window shall be opened on the wall or "partition" between the inside and outside of the separation to serve as the necessary passage for transferring the articles inside and outside during the inoculation process, so as to reduce the number of people entering and leaving the inside and reducing the pollution degree. The small windows are 60cm wide, 40cm high and 30cm thick, with opposite sashes hung inside and outside.
(4) the volume of sterile room is small and tight. After using for a period of time, the indoor temperature is very high, so ventilation window should be set. The ventilation window shall be set on the ceiling at the entrance of the inner room (i.e. far away from the workbench Zui). Zui is a double-layer structure, the outer layer is a shutter, and the inner layer can be a shutter. The ventilation window can be opened after use in the inner chamber and before sterilization to circulate air. If possible, it can install constant temperature and humidity machine.
2. Sterile indoor equipment and appliances
(1) no matter what the material and purpose of the working table in the sterile room, it is required to have a smooth surface and a level surface.
(2) one UV lamp (mostly 30W) shall be installed in the inner chamber and the outer chamber respectively. The ultraviolet lamp in the inner chamber shall be installed directly above the frequently working seat, 2m from the ground, and the ultraviolet lamp in the outer chamber can be installed in the center of the outer chamber.
(3) the outer rooms should have special work clothes, shoes, caps, masks, pottery basins and towels containing the suer water, hand held sprayers and 5% carbolic acid solutions.
(4) the interior room shall be equipped with alcohol lamp, common inoculation tools, stainless steel knife, scissors, tweezers, 70% alcohol cotton ball, industrial alcohol, glass plate, special crayon, record book, pencil, label paper, glue, waste basket, etc.
3. Sterilization and disinfection of sterile room
(1) fumigation: This is a measure for complete sterilization in the sterile room. When the sterile room has been used for a long time and the pollution is serious, fumigation and sterilization should be carried out. It can be fumigated with formaldehyde, lactic acid or sulfur.
(2) spray: before the use of asepsis room. Spray can cause particles and microorganisms to settle in the air, preventing dust flying on the desktop and ground, and having bactericidal effect. It can be sprayed with 5% carbolic acid.
(3) ultraviolet irradiation: before each use of sterile room. UV has a good bactericidal effect. Generally, the ultraviolet lamp should be turned on for 30-60min.
4. Sterile room work procedures
(1) sterilization in sterile room. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5% carbolic acid spray in the interior and exterior chambers.
(2) after washing hands with soap, move the required equipment into the outer room; put on sterilized work clothes, work caps and work shoes in the outer room, wear masks, and then wash hands with 2% coal phenol soap solution for 2 minutes.
(3) move all necessary articles into the inner room for inventory and position. Spray the 5% carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5 ~ 10min.
(4) before inoculation operation, wipe hands with 70% alcohol cotton ball; when aseptic operation is carried out, the action shall be gentle, and air fluctuation and ground dust shall be minimized.
(5) pay attention to safety in work. If the cotton plug catches fire, hold it tightly or wrap it with a wet cloth to extinguish it. Do not blow it with your mouth, so as not to expand the combustion. If the bacteria culture is spilled or smashed into the bacteria container, wrap it with a rag soaked with 5% carbolic acid, wipe the table or ground with a rag soaked with 5% carbolic acid, and then continue the operation after wiping your hands with an alcohol cotton ball.
(6) at the end of the work, clean up the table immediately, and remove all the items and wastes that should not be stored in the asepsis room, then irradiate 30min with 5% carbolic acid spray or ultraviolet lamp.
(IV) constant temperature culture room
1. Setting of culture room
(1) there should be two rooms inside and outside the culture room. The inner room is the culture room and the outer room is the buffer room. The room volume should not be large to facilitate air sterilization. The area of the inner room is about 3.2 × 4.4 = 14m2, the area of the outer room is about 3.2 × 1.8 = 6m2, and the height is about 2.5m. All rooms should have ceilings.
(2) the upper part of the wall separating the inner chamber and the outer chamber shall be provided with air vent with air filter device.
(3) in order to meet the temperature requirements of microorganisms, a constant temperature and humidity machine shall be installed.
(4) ultraviolet lamp shall be installed in the center of the room for sterilization.
2. Indoor equipment and appliances
(1) the inner chamber is usually equipped with a culture stand and a bottle shaker (shaker). There are two kinds of commonly used bottle Shakers: rotary and reciprocating.
(2) the outer room should have special work clothes, shoes, caps, masks, hand held sprayers and 5% carbolic acid solutions, 70% alcohol cotton balls and so on.
3. Sterilization and disinfection of culture room
The same as the sterilization and disinfection measures in the sterile room.
Small scale culture can be carried out in constant temperature incubator instead of constant temperature incubator.
Shandong ed purification Engineering Co., Ltd. is a professional manufacturer of laboratory planning and design. It has been engaged in laboratory decoration, laboratory design, laboratory transformation and other businesses for many years. The company has a professional laboratory design team, covering the laboratory decoration construction team. Construction of microorganism clean laboratory design of microorganism clean laboratory decoration of microorganism clean laboratory planning of microorganism clean laboratory, looking for purification. For more knowledge of medical purification industry, please pay attention to WeChat public address: Aide-188 (Ed purify oxygen supply).