Clean operating room air concentration of bacteria detection method
1. Sampling conditions
      Air conditioning system in the open state, the concentration of bacteria should be detected in other projects completed, the whole surface of the chamber after routine disinfection. Density of surface contamination monitoring program, according to the method "sterilize hospital hygiene standards" GB15982-1995 testing, the test results shall be in accordance with Table 3. Sampling point settings, when outlet centralized arrangement, coping surgical area and the surrounding area were detected; when the outlet scattered arrangement, the whole room uniform testing. Measuring points principle, centralized air condition, where the operating room are used 3:00 swatches; Class I operating room support areas namely swatches using 8:00 2:00 every side; grade Ⅱ operating room Auxiliary Area 6:00 swatches That 2:00 every long side; grade Ⅲ operating room Auxiliary Area 4:00 swatches. When the outlet scattered arrangement, according to the whole room unified fabric detection, measuring points can be uniform, but not to be arranged directly beneath the outlet.
2. Sampling Method
      Planktonic bacteria concentrations measured using the floating method. Dust concentration measurement points and measuring points should be measured in the same area, each sampling time should not exceed 30 min. Settlement bacterial concentration measured by the sedimentation method, measurement points both to the test area of not less than dust concentration measurement points, but also to meet the requirements specified minimum number of petri dishes. There must be two times the control sampling. 1st and dish do comparison tests for the detection of each batch of a control plate. The second is in the detection, per room or per region a control plate.
3. Sampling Height:
      Sampling point may be arranged on the ground or above the ground of any height 0.8m; detecting the operating table in the surgical area should no; when the operating table has been fixed, height should be above the table 0.25cm.
4. Sampling Considerations
      Place the sample from the total area of the flat innermost room started to assign. Laying out successively, each room is from the innermost room layout starting position. The last point is arranged near the door; the reverse order when the charge plate, from the outermost of the room began to collect, per room starting from the innermost closing the door, the first arrangement of the dish and finally closed; uniform distribution within the clean room; negative control flat. Note laminar flow clean room should try to avoid the efficient delivery outlet directly below, if fixed equipment such as operating table, etc., can be placed on the device.